Scientific Papers

Over the last 10 years, the Virtopsy® team has published around 400 peer-reviewed scientific articles.

Zora

2026

  1. Advances in wound ballistic research: Computed tomography analysis of projectile effects in simulantsCrossref:10.1016/j.jflm.2025.103061 DOI
  2. Assessing the impact of bone type on the accuracy of methylation-based forensic age estimationCrossref:10.1016/j.fsigen.2026.103459 DOI
  3. Attenuating trauma- and cocaine-related intrusions by blocking memory reconsolidation with minocycline: protocol for a transdiagnostic randomized controlled trialCrossref:10.1080/20008066.2026.2650920 DOI
  4. Challenges in mRNA-based time since deposition estimation: A multiplex MPS primer panel investigationCrossref:10.1016/j.fsigen.2025.103415 DOI
  5. Comparison of human metabolome changes identified in a placebo-controlled amphetamine administration study versus those using forensic toxicology routine dataCrossref:10.1038/s41598-026-34985-w DOI
  6. Conversion to publication rate of abstracts presented at annual congresses of the International Society of Forensic Radiology and ImagingCrossref:10.1016/j.fri.2025.200661 DOI
  7. Dorsal hand image comparison: A survey of image comparison practitionersCrossref:10.1016/j.forsciint.2025.112758 DOI
  8. Fahreignungsuntersuchungen bei Senioren am IRM-UZH – eine retrospektive Analyse
  9. Fatal intoxication involving amphetamine, clonazafone, a benzodiazepine prodrug, and fluoro-etonitazene, a new synthetic opioidCrossref:10.1007/s00414-025-03627-7 DOI
  10. First experience with 0.31 Tesla low-field MRI in post-mortem fetal examinationsCrossref:10.1007/s00414-025-03698-6 DOI
  11. Forensic 3D avatars – AI-assisted injury detection and interactive digital-twin visualizationCrossref:10.1007/s12024-026-01200-1 DOI
  12. From 3D Surface Documentation to Visualization in Forensic Practice: A Review of Current Methods and Emerging TechnologiesPubMed:PMID:42030038 DOI
  13. From Unsupervised Selection of AIMs to Likelihood-Based BGA inference: Challenges Revealed by OpenADMIXTURE and GENOGEOGRAPHERCrossref:10.1016/j.fsigen.2026.103433 DOI
  14. High-resolution bone imaging and spectral soft-tissue contrast in post-mortem photon-counting CT DOI
  15. How do substance and polysubstance use trajectories differ by sexual attraction from ages 17 to 24? A community-based longitudinal cohort study in SwitzerlandCrossref:10.1136/bmjph-2025-003583 DOI
  16. In Situ pH Determination Using <sup>1</sup> H‐MRS Acetate Chemical Shift for Noninvasive Postmortem ExaminationsCrossref:10.1002/nbm.70323 DOI
  17. Intracranial needle insertion into an infant’s brain: a case report revealing an unprecedented computed tomography discoveryCrossref:10.1007/s00414-025-03685-x DOI
  18. Memory deficits of MDMA users are linked to cortical thinning related to 5-HT receptor densitiesCrossref:10.1093/brain/awaf391 DOI
  19. Methylation-based forensic age estimation on different biogeographic backgrounds: A study on Central Europeans, East Asians and West AfricansCrossref:10.1016/j.fsigen.2025.103397 DOI
  20. Musculoskeletal Diseases 2026–2029Crossref:10.1007/978-3-032-17040-8 DOI
  21. Myocardial calcification revealed by postmortem computed tomography in a case of infantile myocardial ischemia caused by anomalous origin of the left coronary artery from the pulmonary arteryCrossref:10.1016/j.legalmed.2025.102743 DOI
  22. Peptide ratios for post-mortem interval estimation using targeted liquid chromatography triple quadrupole mass spectrometryCrossref:10.1007/s00414-025-03693-x DOI
  23. Perceived Chronic Stress prior to SARS-CoV-2 Infection Predicts Ongoing Symptomatic COVID-19: A Prospective Cohort StudyCrossref:10.1159/000547858 DOI
  24. Petrous bone fractures and ossicular chain lesions detected by PMCT in burnt bodiesCrossref:10.1016/j.forsciint.2025.112709 DOI
  25. Photography techniques for 3D surface reconstruction during autopsyCrossref:10.1016/j.forsciint.2025.112702 DOI
  26. Something is not nothing: Hair-tested substance use and cognitive functions in a large community sample of young adultsCrossref:10.1192/j.eurpsy.2026.10156 DOI
  27. Stereotactic rodent-to-human approximation of the mesencephalic cuneiform nucleus to guide deep brain stimulationPubMed:PMID:41786287 DOI
  28. Striking energy achieved by knee strikes, elbow blows, head butts and fist punchesCrossref:10.1016/j.forsciint.2026.112889 DOI
  29. The Impact and Reliability of Tissue Segmentation on In Vivo Magnetic Resonance Spectroscopy Metabolite QuantificationCrossref:10.1002/mrm.70380 DOI
  30. Think Forensic, Act Forensic, Feel Forensic: Qualitative Descriptive Recording of Forensic Nurses’ Experiences in German-Speaking SwitzerlandCrossref:10.1097/jfn.0000000000000576 DOI
  31. Where i got to – in radiologyCrossref:10.1067/j.cpradiol.2026.04.011 DOI

2025

  1. A 28-Year-Old Man With Consecutive Gastrointestinal Symptoms, Kidney Failure, and Progressive Neurologic DeteriorationCrossref:10.1016/j.chest.2025.05.031 DOI
  2. A CE-based mRNA profiling method including six targets to estimate the time since deposition of blood stains DOI
  3. Accuracy and Robustness of Bone Volume Fraction Assessment by Photon-Counting, Dual-Energy, and Quantitative CT using Micro-CT as Standard of Reference DOI
  4. Analysis of <i>Treponema pallidum</i> subsp. <i>pallidum</i> predicted outer membrane proteins (OMPeomes) in 21 clinical samples: variant sequences are predominantly surface-exposedCrossref:10.1128/msphere.00213-25 DOI
  5. Assessing the Feasibility of In Vitro Assays in Combination with Biological Matrices to Screen for Endogenous CYP450 Phenotype Biomarkers Using an Untargeted Metabolomics Approach—A Proof of Concept StudyCrossref:10.3390/metabo15120791 DOI
  6. Assessing transcriptomic signatures of aging: Testing an mRNA marker panel for forensic age estimation of blood samples DOI
  7. Assessment of cardiac biomarker “point-of-care” testing as postmortem diagnostic tool DOI
  8. Association between inflammatory biomarkers, chronic stress, and pericoronary adipose tissue attenuation obtained with coronary CTPubMed:PMID:40886153 DOI
  9. Associations Between Self‐Reported Cocaine Use Patterns and Cocaine and Its Metabolites in Hair: Implications for Clinical and Forensic Practices DOI
  10. Automated volumetric estimation of six basic thoracic and abdominal organs in postmortem computed tomography data using deep learning techniques DOI
  11. Ayahuasca-inspired DMT/HAR formulation reduces brain differentiation between self and other faces DOI
  12. Cannabis use is associated with changes in psychological and functional well-being during young adulthood: evidence from self-reports and hair analysesCrossref:10.1017/s003329172510144x DOI
  13. Clinically Uncertain Liver Masses: A Guide to Distinguishing Poorly Differentiated Primary Liver CancerCrossref:10.3390/biomedicines13051063 DOI
  14. Co-Boost: boosting and guiding neuroplasticity by combining ketamine with neurofeedback-assisted learning-towards an individualised and integrated pharmaco-psychotherapy for cocaine addiction: study protocol for a randomised, placebo-controlled, double-blind, parallel-group, single-centre trialPubMed:PMID:40999531 DOI
  15. Colchicum autumnale poisoning in a dairy farm in Switzerland: a glimpse at the milk safety aspectCrossref:10.4081/ijfs.2025.13828 DOI
  16. Comparative evaluation of emphysema quantification: Standardized %LAV−950 versus DL-based emphysema quantification with clinical parameter correlationCrossref:10.1097/md.0000000000046246 DOI
  17. Correlation of pulmonary fat embolism with trauma and resuscitation in children DOI
  18. Delayed Ferumoxtran‐10‐Enhanced Magnetic Resonance Neurography of the Lumbosacral Plexus: Impact on Vascular Suppression and Image Quality DOI
  19. Development and thorough evaluation of a multi-omics sample preparation workflow for comprehensive LC-MS/MS-based metabolomics, lipidomics and proteomics datasets DOI
  20. Dynamic ultrasound guided assessment of pelvic ring injury measurement (PRIME) for traumatic symphysis diastasisPubMed:PMID:41148366 DOI
  21. Effects of Subanesthetic Oromucosal Dexmedetomidine on Sleep in Humans: A Randomized, Controlled Pharmacokinetics–Pharmacodynamics StudyCrossref:10.1097/aln.0000000000005314 DOI
  22. Endocannabinoid and steroid hormone levels during and after pregnancy in fingernail samples from mothers and their infantsCrossref:10.1016/j.steroids.2025.109663 DOI
  23. Evaluation of Bladder Wash as an Alternative Specimen for Postmortem Toxicology: Comparison to Screening Results of Urine and Kidney Tissue DOI
  24. Evaluation of Therapeutic Opioids in Hair of Neonatal and Pediatric PatientsCrossref:10.1002/dta.3935 DOI
  25. Exploring transcriptomic signatures in sudden unexplained death (SUD) cases DOI
  26. Feasibility and usefulness of in-hospital and at-home salivary sampling in healthy dogs and trilostane-treated dogs with Cushing’s syndrome: a prospective observational studyPubMed:PMID:41131585 DOI
  27. FORMics: Forensic Microbiome and Metabolome Analysis for Body Fluid Identification
  28. From Promise to Practice: Why HRMS Has Yet to Fully Revolutionize Forensic Toxicology DOI
  29. Genetic Testing of a High-End ‘Angel Skin’ Precious Coral Necklace Identifies a Species New to the Precious Coral Trade and Potentially New to ScienceCrossref:10.3390/d17060395 DOI
  30. ISFRI Education Working Group: competencies and training requirements for reporting and interpretation of adult forensic post-mortem imagingCrossref:10.1016/j.fri.2025.200652 DOI
  31. ISFRI guidelines working group: best practice standards for non-contrast postmortem computed tomography (PMCT) of blunt force traumaCrossref:10.1016/j.fri.2025.200630 DOI
  32. Kategorisierte Analytische Fünfjahresintervalluntersuchung von Sexualdelikten am IRM UZH DOI
  33. Left ventricular perforation resistance during pleural drainage puncture DOI
  34. Magnetic resonance imaging of regional gray matter volume in persons who died by suicide DOI
  35. Microbial and seminal traces of sexual intercourse and forensic implicationsCrossref:10.1186/s40168-025-02268-7 DOI
  36. mRNA profiling and donor association of mock casework samples: Results of a 3rd and 4th EDNAP collaborative exercise DOI
  37. Multianalyte Approach-Including Automated Preparation of Calibrators-for Validated Quantification of 82 Drugs in Whole Blood by Liquid Chromatography-Tandem Mass Spectrometry DOI
  38. National Consensus on the Assessment of Visual Function for Driving in Switzerland DOI
  39. New Approaches To Identify Urine and Hair Adulteration Attempts in Forensic Toxicology: A Proof-of-Concept Study Using a Proteomics Approach Based on Liquid Chromatography–Mass Spectrometry (LC-MS)Crossref:10.1021/acs.analchem.5c03096 DOI
  40. Pars basilaris size to estimate fetal and young infant age using forensic post mortem CT imaging DOI
  41. Postmortem computed tomography-based evaluation of brain decomposition: Correlation with postmortem interval and cause-of-death determinabilityCrossref:10.1016/j.legalmed.2025.102710 DOI
  42. Postmortem CT and MRI for detecting bowel obstruction in cases of pediatric sepsis deaths DOI
  43. Postmortem CT: Applications in Clinical and Forensic Medicine DOI
  44. Prevalence and origin of prominent nutrient channels of the ilium bone on MR-imaging DOI
  45. Prolonged Detection of GHB Intake in Urine: Are We Finally There?Crossref:10.1002/dta.3956 DOI
  46. Psychobiological stress outcomes in mothers, fathers, and children after successful assisted reproductionCrossref:10.1080/02646838.2025.2596046 DOI
  47. Quantitative wound ballistic analysis of gelatin head phantoms by computed tomography using the total crack length method DOI
  48. Radial EBUS to Identify Tuberculosis Mediastinal Lymph Nodes for Biopsy in Young Children DOI
  49. RNA Profiling for Forensic Applications: Body Fluid Identification, Estimating the Age of Stains, Determining the Age of the Stain Donor
  50. Sample Preparation for Multi‐Omics Analysis: Considerations and Guidance for Identifying the Ideal Workflow DOI
  51. Standardizing a microbiome pipeline for body fluid identification from complex crime scene stains DOI
  52. Swiss recommendations on driving ability in patients with diabetes mellitusCrossref:10.57187/s.4665 DOI
  53. Systematic review and comparison of measurement methods for determining the cardiothoracic ratio (CTR) on chest X-rays with subsequent validation using chest CTCrossref:10.1097/md.0000000000045062 DOI
  54. TASER 7 and TASER 10 Probe Penetration Into Human Skull Simulants DOI
  55. The current state of forensic imaging – perspectives DOI
  56. The current state of forensic imaging – post mortem imaging DOI
  57. The current state of forensic imaging – recommended radiological tools and international guidelines DOI
  58. The current state of forensic imaging– clinical forensic imaging DOI
  59. The evolution of postmortem investigation: a historical perspective on autopsy’s decline and imaging’s role in its revival DOI
  60. The influence of cancer on a forensic age estimation toolCrossref:10.18632/aging.206281 DOI
  61. The periaqueductal gray in chronic low back pain: dysregulated neurotransmitters and function DOI
  62. Toward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography–High‐Resolution Mass Spectrometry (GC‐HRMS) and Liquid Chromatography–High‐Resolution Tandem Mass Spectrometry (LC‐HRMS/MS) DOI
  63. Trace DNA from a century-old holotype specimen resolves taxonomic uncertainties: the case of the Hawaiian pink precious coral (Pleurocorallium secundum), a CITES-listed species used in jewelryCrossref:10.1007/s00338-025-02678-x DOI
  64. Uncovering genetic signatures of the Walser migration in the Alps: Patterns of diversity and differentiation DOI
  65. Untargeted Metabolomics for Forensic Body Fluid Identification-A Pilot StudyCrossref:10.1021/acs.analchem.5c04864 DOI
  66. Using Hologram-Based Augmented Reality in Anatomy Learning: The TEACHANATOMY Randomized Trial DOI
  67. UYSD: a novel data repository accessible via public website for worldwide population frequencies of Y-SNP haplogroups DOI
  68. VIRTual autOPSY—applying CT and MRI for modern forensic death investigationsCrossref:10.3389/fradi.2025.1557636 DOI
  69. Virtual Bullet Examination: Forensic Insights from CT Imaging in Gunshot VictimsCrossref:10.3390/forensicsci5020023 DOI

Pubmed

Click here for a list of all Pubmed listed articles

Ossification variation of the medial clavicular epiphysis in children, adolescents and young adults.

Engler P, Kruk K, Graf N, Stinn B, Wildermuth S, Leschka S, Falkowski AL, Waelti S, Dietrich TJ, Fischer TS.Radiography (Lond). 2025 Aug;31(5):103106. doi: 10.1016/j.radi.2025.103106. Epub 2025 Jul 30.PMID: 40749420
In case of disagreement, a third senior radiologist and head of forensic imaging, resolved discrepancies. Gender differences and diagnostic accuracy between 0.75 mm and 1.5 mm CT slice thickness were assessed. …

Postmortem CT: Applications in Clinical and Forensic Medicine.

Solomon N, Elifritz J, Adolphi NL, Decker SJ, Filograna L, Kroll JJF, Gascho D, Thali MJ, Gosangi B, Sanchez H, Revzin MV, Sinusas AJ.Radiographics. 2025 Jun;45(6):e240192. doi: 10.1148/rg.240192.PMID: 40372934 Free PMC article. Review.
The authors introduce the reader, particularly the radiologist, to the growing subspecialty of forensic imaging (focusing on imaging of the deceased), specifically focusing on PMCT and its applications in death investigation in both clinical and forensic settings. . …

The current state of forensic imaging – recommended radiological tools and international guidelines.

Dedouit F, Ducloyer M, Elifritz J, Adolphi NL, Yi-Li GW, Decker S, Ford J, Kolev Y, Thali M.Int J Legal Med. 2025 Sep;139(5):2219-2231. doi: 10.1007/s00414-025-03465-7. Epub 2025 Mar 25.PMID: 40128331 Free PMC article. Review.
The last few decades have seen the emergence of forensic imaging, both clinical and post-mortem. Year after year, the scientific community has refined the radiological tools that can be used for post-mortem and clinical forensic purposes. As a result, scientific soc …

The current state of forensic imaging – post mortem imaging.

Dedouit F, Ducloyer M, Elifritz J, Adolphi NL, Yi-Li GW, Decker S, Ford J, Kolev Y, Thali M.Int J Legal Med. 2025 May;139(3):1141-1159. doi: 10.1007/s00414-025-03461-x. Epub 2025 Mar 24.PMID: 40126650 Free PMC article. Review.
Over the last few decades, forensic imaging has become an essential part of current forensic practice. The aim of this 4-part review is to provide a comprehensive overview of forensic imaging over the first 25 years of this century. …

The current state of forensic imaging – perspectives.

Dedouit F, Ducloyer M, Elifritz J, Adolphi NL, Yi-Li GW, Decker S, Ford J, Kolev Y, Thali M.Int J Legal Med. 2025 Nov;139(6):2819-2827. doi: 10.1007/s00414-025-03466-6. Epub 2025 Mar 21.PMID: 40116908 Free PMC article. Review.
This fourth part of the review of the current state of forensic imaging describes the future potential influence of artificial intelligence in forensic imaging. In addition to this important point, training in forensic imaging is discusse …

The current state of forensic imaging– clinical forensic imaging.

Dedouit F, Ducloyer M, Elifritz J, Adolphi NL, Yi-Li GW, Decker S, Ford J, Kolev Y, Thali M.Int J Legal Med. 2025 Jul;139(4):1639-1646. doi: 10.1007/s00414-025-03464-8. Epub 2025 Mar 18.PMID: 40100352 Free PMC article. Review.
Clinical forensic imaging could be defined as the use of imaging first realised for medical care as evidence for a judicial purpose. …

Photon-counting CT for forensic death investigations-a glance into the future of virtual autopsy.

Gascho D.Front Radiol. 2024 Sep 16;4:1463236. doi: 10.3389/fradi.2024.1463236. eCollection 2024.PMID: 39351027 Free PMC article.
The article discusses the technical principles of photon-counting CT detectors and highlights its potential applications in forensic imaging, including high-resolution virtual autopsies, pediatric forensic CT, trauma analysis, and bone density measurements. …

CT-based sex estimation on human femora using statistical shape modeling.

Fliss B, Luethi M, Fuernstahl P, Christensen AM, Sibold K, Thali M, Ebert LC.Am J Phys Anthropol. 2019 Jun;169(2):279-286. doi: 10.1002/ajpa.23828. Epub 2019 Mar 30.PMID: 30927271
MATERIALS AND METHODS: For this study, we acquired a total of 61 femora from routine postmortem CT scans at the Institute for Forensic Medicine of the University of Zurich. The femora were extracted using segmentation technique. After building a SSM, we used the linear reg …

Standardized medical image registration for radiological identification of decedents based on paranasal sinuses.

Gascho D, Philipp H, Flach PM, Thali MJ, Kottner S.J Forensic Leg Med. 2018 Feb;54:96-101. doi: 10.1016/j.jflm.2017.12.003. Epub 2018 Jan 10.PMID: 29348074
Due to the increasing use of computed tomography (CT) for head examinations in clinical radiology and the increased use of postmortem CT (PMCT) in forensic imaging, the comparison of three-dimensional (3D) clinical CT (termed as antemortem CT (AMCT) in this article) …

Death by “Snow”! A Fatal Forensic Case of Cocaine Leakage in a “Drug Mule” on Postmortem Computed and Magnetic Resonance Tomography Compared With Autopsy.

Flach PM, Gascho D, Fader R, Martinez R, Thali MJ, Ebert LC.Am J Forensic Med Pathol. 2017 Dec;38(4):339-344. doi: 10.1097/PAF.0000000000000336.PMID: 28737525 Free article.
This forensic case presents unique postmortem imaging of a “drug mule” with fatal intoxication due to cocaine leakage on postmortem computed and magnetic resonance (MR) tomography compared with autopsy.Imaging by postmortem computed and MR tomography was performed before autopsy, …

Gland segmentation in colon histology images: The glas challenge contest.

Sirinukunwattana K, Pluim JPW, Chen H, Qi X, Heng PA, Guo YB, Wang LY, Matuszewski BJ, Bruni E, Sanchez U, Böhm A, Ronneberger O, Cheikh BB, Racoceanu D, Kainz P, Pfeiffer M, Urschler M, Snead DRJ, Rajpoot NM.Med Image Anal. 2017 Jan;35:489-502. doi: 10.1016/j.media.2016.08.008. Epub 2016 Sep 3.PMID: 27614792 Free article. Review.
Baumeister R, Mauf S, Laberke P, Krupp A, Thali MJ, Flach PM.Forensic Sci Med Pathol. 2015 Dec;11(4):589-95. doi: 10.1007/s12024-015-9716-2. Epub 2015 Oct 5.PMID: 26434653 Free article.
The objective of this case report was the visualization and evaluation of unique electrocution-related injuries by postmortem contrast and non-contrast enhanced imaging compared to a forensic autopsy. METHODS: Forensic imaging included whole-body postmortem computed …

Scopus

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Books

Gil Brogdon”s standard textbook “Brogdon’s Forensic Radiology” (CRC Press, Boca Raton, USA, 2nd ed. 2010) which was edited by Gil Brodon, Michael Thali and Mark Viner provides an excellent overview of forensic radiology and features several chapters on Virtopsy®. Click here to order the book online through Amazon.com

The textbook “The Virtopsy Approach” (CRC Press, Boca Raton, USA, 1st ed. 2009) which was edited by Michael Thali, Richard Dirnhofer and Peter Vock provides in-depth information on forensic imaging.Click here to order the book online through Amazon.com

News articles

There are numerous articles in the popular press on Virtopsy® and Forensic Imaging. A selection of articles is provided here: